What's the difference between just the mixture of biomolecules and living cells?

I'm making experiments here in UCSF and sometimes remember Fujiwara-san, who was a mentor of BIOMOD Team Sendai in 2012 and 2013. He was very strict about not only experiments but also the management of the team (though I liked him a lot!). I wondered what he was doing now and found his recent paper (review?).

Kei Fujiwara, Miho Yanagisawa, Shin-ichiro M. Nomura. 'Reconstitution of intracellular environments in vitro and in artificial cells.' BIOPHYSICS
Vol. 10 (2014) p. 43-48

https://www.jstage.jst.go.jp/article/biophysics/10/0/10_43/_article

This review is about the challenge of reconstitution of cells from the mixture of E.coli extract and GUV (giant unilamellar vesicles. In this review it represents the model of cell membrane). The underlying question is- what's the difference between just the mixture of biomolecules (combination of intracellular extract and cell membrane) and living cells? Many scientist believe that life started from the encapsulation of everything necessary for self-reproduction in lipid vesicle and that reproducing the encapsulation in a certain, suitable condition would yield the reconstruction of life itself.

To mimic the natural intracellular environment as much as possible, the authors took two approaches: 1. condensing cell extract as much as it reaches the similar concentration as intracellular matrix of living cells. 2. making the extract additive (salt) free. 

In the end they succeeded in making additive free cell extract (AFCE). On the other hand, though they tried to make the concentration of AFCE similar to those of intracellular matrix, the AFCE didn't bring efficient protein synthesis. The authors suggest the necessity of additional trials of expressing membrane proteins, which would make the artificial cells more like real cells, in GUV.